MSTN (Human) Recombinant Protein
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Specification
Product Description
Human MSTN recombinant protein expressed in Escherichia coli.
Sequence
MNENSEQKENVEKEGLCNACTWRQNTKSSRIEAIKIQILSKLRLETAPNISKDVIRQLLPKAPPLRELIDQYDVQRDDSSDGSLEDDDYHATTETIITMPTESDFLMQVDGKPKCCFFKFSSKIQYNKVVKAQLWIYLRPVETPTTVFVQILRLIKPMKDGTRYTGIRSLKLDMNPGTGIWQSIDVKTVLQNWLKQPESNLGIEIKALDENGHDLAVTFPGPGEDGLNPFLEVKVTDTPKRSRR
Host
Escherichia coli
Theoretical MW (kDa)
26.8
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Endotoxin Level
< 0.1 EU/ug
Activity
The activity is determined by the ability to inhibit 50 ng/mL of Myostatin activity on MPC-11 cells. The expected ED50 for this effect is 0.13-0.2 ug/mL.
Storage Buffer
No additive
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 5 mM acetic acid, store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — MSTN
Entrez GeneID
2660Protein Accession#
O14793Gene Name
MSTN
Gene Alias
GDF8
Gene Description
myostatin
Omim ID
601788Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. This gene is thought to encode a secreted protein which negatively regulates skeletal muscle growth. [provided by RefSeq
Other Designations
growth differentiation factor 8
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