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Last updated: 2019/8/18

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Casp3 (Active form) (Mouse) Recombinant Protein BioActive

  • Catalog # : P4485
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  • Specification
  • Product Description:
  • Mouse Casp3 (Active form) (P29452) recombinant protein expressed in Escherichia coli.
  • Host:
  • Escherichia coli
  • Theoretical MW (kDa):
  • large(17)+small(11)
  • Form:
  • Lyophilized
  • Preparation Method:
  • Escherichia coli expression system
  • Activity:
  • 300,000 units/mg
  • Storage Buffer:
  • No additive
  • Storage Instruction:
  • Store at -80°C on dry atmosphere.
    Reconstitute to 1 unit per uL in PBS containing 15% glycerol. After reconstitution with PBS, the enzyme should be aliquoted and immediately stored at -80°C.
    Aliquot to avoid repeated freezing and thawing.
    Centrifuge the vial prior to opening.
  • Note:
  • Unit defination: One unit of the recombinant caspase-3 is the enzyme activity that cleaves 1 nmol of the caspase substrate DEVD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% glycerol, and 10 mM DTT.
    Since this is an active protein, the molecular weight of the large subunit is 17 kDa and small is 11 kDa.
  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Gene Name:
  • Casp3
  • Gene Alias:
  • A830040C14Rik,Apopain,CC3,CPP32,Caspase-3,Lice,Yama
  • Gene Description:
  • caspase 3
  • Other Designations:
  • caspase 3, apoptosis related cysteine protease
  • Interactome
  • Interactome
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