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TNFSF11 (Human) Recombinant ProteinBioActive

  • Catalog # : P4434
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  • Specification
  • Product Description:
  • Human TNFSF11 (O14788) recombinant protein expressed in Escherichia coli.
  • Sequence:
  • Host:
  • Escherichia coli
  • Theoretical MW (kDa):
  • 20
  • Form:
  • Lyophilized
  • Preparation Method:
  • Escherichia coli expression system
  • Endotoxin Level:
  • < 0.1 EU/ug
  • Activity:
  • The activity is determined by the ability to induce a dose-dependent stimulation of IL-8 production by human PBMCs. The expected ED50 for this effect is 39-51 ng/mL.
  • Quality Control Testing:
  • 1 ug/lane in 4-20% Tris-Glycine gel Stained with Coomassie Blue

    QC Testing of P4434
    Lane 1: non-reducing conditions
    Lane 2: reducing conditions
  • Storage Buffer:
  • Lyophilized from 10 mM Na2PO4, pH 8.0
  • Storage Instruction:
  • Store at -20°C on dry atmosphere.
    After reconstitution with sterilized water, store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis
  • Human PBMCs were cultured with 0 to 1000 ng/mL human TNFSF11. Human IL8 production was measured after 48 hours and the linear portion of the curve was us used to calculate the ED50.

  • Applications
  • Functional Study
  • Application Image
  • Functional Study
  • Gene Information
  • Entrez GeneID:
  • 8600
  • Gene Name:
  • TNFSF11
  • Gene Alias:
  • Gene Description:
  • tumor necrosis factor (ligand) superfamily, member 11
  • Gene Summary:
  • This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000178585,TNF-related activation-induced cytokine,osteoclast differentiation factor,osteoprotegerin ligand,receptor activator of nuclear factor kappa B ligand,tumor necrosis factor ligand superfamily, member 11
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