The activity is determined by an agarose microdroplet assay using?human U937 cells as?targets and is 0.5-1 ug/mL and the production of IL-8 by human PBMCs. The expected ED50 for this effect is 88-132 ng/mL.
Quality Control Testing:
1 ug/lane in 4-20% Tris-Glycine gel Stained with Coomassie Blue
Lane 1: non-reducing conditions Lane 2: reducing conditions
Lyophilized from 10 mM Na2PO4, pH 7.5
Store at -20°C on dry atmosphere. After reconstitution with sterilized water, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq