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Last updated: 2017/11/12

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MIF (Human) Recombinant ProteinBioActive

  • Catalog # : P4426
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  • Specification
  • Product Description:
  • Human MIF (AAA36315) recombinant protein expressed in Escherichia coli.
  • Sequence:
  • MPMFIVNTNVPRASVPDGFLSELTQQLAQATGKPPQYIAVHVVPDQLMAFGGSSEPCALCSLHSIGKIGGAQNRSYSKLLCGLLAERLRISPDRVYINYYDMNAANVGWNNSTFA
  • Host:
  • Escherichia coli
  • Theoretical MW (kDa):
  • 12.5
  • Form:
  • Lyophilized
  • Preparation Method:
  • Escherichia coli expression system
  • Endotoxin Level:
  • < 0.1 EU/ug
  • Activity:
  • The activity is determined by an agarose microdroplet assay using?human U937 cells as?targets and is 0.5-1 ug/mL and the production of IL-8 by human PBMCs. The expected ED50 for this effect is 88-132 ng/mL.
  • Quality Control Testing:
  • 1 ug/lane in 4-20% Tris-Glycine gel Stained with Coomassie Blue

    QC Testing of P4426
    Lane 1: non-reducing conditions
    Lane 2: reducing conditions
  • Storage Buffer:
  • Lyophilized from 10 mM Na2PO4, pH 7.5
  • Storage Instruction:
  • Store at -20°C on dry atmosphere.
    After reconstitution with sterilized water, store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 4282
  • Gene Name:
  • MIF
  • Gene Alias:
  • GIF,GLIF,MMIF
  • Gene Description:
  • macrophage migration inhibitory factor (glycosylation-inhibiting factor)
  • Gene Summary:
  • This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq
  • Other Designations:
  • glycosylation-inhibiting factor,phenylpyruvate tautomerase
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