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CD40LG (Human) Recombinant ProteinBioActive

  • Catalog # : P4377
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  • Specification
  • Product Description:
  • Human CD40LG (P29965) recombinant protein expressed in Escherichia coli.
  • Sequence:
  • MQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLWLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL
  • Host:
  • Escherichia coli
  • Theoretical MW (kDa):
  • 16.3
  • Form:
  • Lyophilized
  • Preparation Method:
  • Escherichia coli expression system
  • Endotoxin Level:
  • < 0.1 EU/ug
  • Activity:
  • The activity is determined by the dose-dependent production of IL-8 from human PBMCs. The expected ED50 for this effect is 8-12 ng/mL.
  • Quality Control Testing:
  • 1 ug/lane in 4-20% Tris-Glycine gel Stained with Coomassie Blue

    QC Testing of P4377
    Lane 1: non-reducing conditions
    Lane 2: reducing conditions
  • Storage Buffer:
  • Lyophilized from 10 mM Na2PO4, pH 7.5
  • Storage Instruction:
  • Store at -20°C on dry atmosphere.
    After reconstitution with sterilized water, store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis
  • Serial dilutions of human CD40LG, starting at 2000 ng/mL, were added to human PBMCs. After 48 hours, cell supernatant was collected and human IL-8 was measured via ELISA and the linear portion of the curve was us used to calculate the ED50.


  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 959
  • Gene Name:
  • CD40LG
  • Gene Alias:
  • CD154,CD40L,HIGM1,IGM,IMD3,T-BAM,TNFSF5,TRAP,gp39,hCD40L
  • Gene Description:
  • CD40 ligand
  • Gene Summary:
  • The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq
  • Other Designations:
  • CD40 antigen ligand,OTTHUMP00000024130,T-B cell-activating molecule,TNF-related activation protein,tumor necrosis factor (ligand) superfamily member 5
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