Il1b (Mouse) Recombinant Protein
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Specification
Product Description
Mouse Il1b (P10749, 118 a.a. - 269 a.a.) partial recombinant protein expressed in Escherichia coli.
Sequence
VPIRQLHYRLRDEQQKSLVLSDPYELKALHLNGQNINQQVIFSMSFVQGEPSNDKIPVALGLKGKNLYLSCVMKDGTPTLQLESVDPKQYPKKKMEKRFVFNKIEVKSKVEFESAEFPNWYISTSQAEHKPVFLGNNSGQDIIDFTMESVSS
Host
Escherichia coli
Theoretical MW (kDa)
17
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
Ion exchange column and HPLC reverse phase column
Purity
> 90% by SDS-PAGE and HPLC
Endotoxin Level
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Activity
The ED50 was determined by the dose-dependent stimulation of thymidine uptake by murine D10S cells is <= 2.0 pg/mL, corresponding to a specific activity of >= 5 x 108 units/mg.
Storage Buffer
Lyophilized from PBS, pH 7.0
Storage Instruction
Store at -20°C on dry atmosphere for 2 years. After reconstitution with deionized water, store at 4°C for 1 month or store at -20°C for 6 months.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — Il1b
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