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Last updated: 2016/12/4

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PRKCE (Human) Recombinant ProteinBioActive

  • Catalog # : P4236
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  • Specification
  • Product Description:
  • Human PRKCE (Q02156) full-length recombinant protein expressed in Sf9 cells.
  • Host:
  • Insect
  • Theoretical MW (kDa):
  • 110
  • Form:
  • Liquid
  • Preparation Method:
  • Insect cell (Sf9) expression system
  • Purity:
  • >= 90% by SDS-PAGE
  • Activity:
  • 1258 nmol phosphate incorporated into PKCepsilon substrate peptide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay).
  • Quality Control Testing:
  • 1.5 ug/lane SDS-PAGE Stained with Coomassie Blue

    QC Testing of P4236
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.25 mM Dithiothreitol, 0.1 mM EGTA, 0.1 mM EDTA, 25% glycerol, 0.1 mM PMSF)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 5581
  • Gene Name:
  • PRKCE
  • Gene Alias:
  • MGC125656,MGC125657,PKCE,nPKC-epsilon
  • Gene Description:
  • protein kinase C, epsilon
  • Gene Summary:
  • Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior. [provided by RefSeq
  • Other Designations:
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