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Last updated: 2017/7/23

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MAPK3 (Human) Recombinant ProteinBioActive

  • Catalog # : P4224
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  • Specification
  • Product Description:
  • Human MAPK3 (P27361) full-length recombinant protein expressed in Sf9 cells.
  • Host:
  • Insect
  • Theoretical MW (kDa):
  • 45
  • Form:
  • Liquid
  • Preparation Method:
  • Insect cell (Sf9) expression system
  • Purity:
  • >= 90% by SDS-PAGE
  • Activity:
  • 383 nmol phosphate incorporated into MBP per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay).
  • Quality Control Testing:
  • 1 ug/lane SDS-PAGE Stained with Coomassie Blue

    QC Testing of P4224
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.25 mM Dithiothreitol, 0.1 mM EGTA, 0.1 mM EDTA, 25% glycerol, 0.1 mM PMSF)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 5595
  • Gene Name:
  • MAPK3
  • Gene Alias:
  • ERK1,HS44KDAP,HUMKER1A,MGC20180,P44ERK1,P44MAPK,PRKM3
  • Gene Description:
  • mitogen-activated protein kinase 3
  • Gene Summary:
  • The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000174538,OTTHUMP00000174540,extracellular signal-regulated kinase 1,extracellular signal-related kinase 1
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