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MAPKAPK2 (Human) Recombinant ProteinBioActive

  • Catalog # : P4220
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  • Specification
  • Product Description:
  • Human MAPKAPK2 (P49137, 46 a.a. - end) partial recombinant protein expressed in Sf9 cells.
  • Host:
  • Insect
  • Theoretical MW (kDa):
  • 68
  • Form:
  • Liquid
  • Preparation Method:
  • Insect cell (Sf9) expression system
  • Purity:
  • >= 90% by SDS-PAGE
  • Activity:
  • 254 nmol phosphate incorporated into MAPKAP kinase 2 substrate peptide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 uM ATP and total of 0.83 uCi/uL P-32.
  • Quality Control Testing:
  • 1 ug/lane SDS-PAGE Stained with Coomassie Blue

    QC Testing of P4220
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.25 mM Dithiothreitol, 0.1 mM EGTA, 0.1 mM EDTA, 25% glycerol, 0.1 mM PMSF)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 9261
  • Gene Name:
  • MAPKAPK2
  • Gene Alias:
  • MK2
  • Gene Description:
  • mitogen-activated protein kinase-activated protein kinase 2
  • Gene Summary:
  • This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000034531,OTTHUMP00000034532
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