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PDK1 (Human) Recombinant ProteinBioActive

  • Catalog # : P4190
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  • Specification
  • Product Description:
  • Human PDK1 (Q15118) full-length recombinant protein expressed in Sf9 cells.
  • Host:
  • Insect
  • Theoretical MW (kDa):
  • 59
  • Form:
  • Liquid
  • Preparation Method:
  • Insect cell (Sf9) expression system
  • Purity:
  • >= 95% by SDS-PAGE
  • Activity:
  • 159 nmol phosphate incorporated into PDKtide substrate peptide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay).
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.25 mM Dithiothreitol, 0.1 mM EGTA, 0.1 mM EDTA, 25% glycerol, 0.1 mM PMSF)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 5163
  • Gene Name:
  • PDK1
  • Gene Alias:
  • -
  • Gene Description:
  • pyruvate dehydrogenase kinase, isozyme 1
  • Gene Summary:
  • Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. [provided by RefSeq
  • Other Designations:
  • mitochondrial pyruvate dehydrogenase kinase isoenzyme 1,pyruvate dehydrogenase kinase, isoenzyme 1
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