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PRKCI (Human) Recombinant ProteinBioActive

  • Catalog # : P4189
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  • Specification
  • Product Description:
  • Human PRKCI (NM_002740) full-length recombinant protein expressed in Sf9 cells.
  • Host:
  • Insect
  • Theoretical MW (kDa):
  • 98
  • Form:
  • Liquid
  • Purity:
  • >= 90% by SDS-PAGE
  • Activity:
  • 664 nmol phosphate incorporated into CREBtide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay in 25uL reaction volume).
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.25 mM Dithiothreitol, 0.1 mM EGTA, 0.1 mM EDTA, 25% glycerol, 0.1 mM PMSF)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 5584
  • Gene Name:
  • PRKCI
  • Gene Alias:
  • DXS1179E,MGC26534,PKCI,nPKC-iota
  • Gene Description:
  • protein kinase C, iota
  • Gene Summary:
  • This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000196630,PRKC-lambda/iota,aPKC-lambda/iota,atypical protein kinase C-lambda/iota,protein kinase C iota type
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