Human GSK3B (P49841) full-length recombinant protein with His tag expressed in Escherichia coli.
Theoretical MW (kDa):
Escherichia coli expression system
>= 90% by SDS-PAGE
Activity of GSK-3beta was determined by an in vitro kinase assay using 100 ng Tau as substrate and 1 ug of the recombinant GSK-3beta in a 20 uL reaction containing 25 mM Tris-HCl, pH 7.5, 5 mM beta-glycerol phosphate, 12 mM MgCl2, 2 mM DTT, 0.1
Lyophilized from 2 mg/mL in 0.1 M NH4CO3
Store at -80°C on dry atmosphere. After reconstitution with PBS containing 15% glycerol, store at -80°C. Aliquot to avoid repeated freezing and thawing.
The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene