Human JUN (P05412) full-length recombinant protein expressed with GST tag in Escherichia coli.
Theoretical MW (kDa):
Escherichia coli expression system
>= 90% by SDS-PAGE
C-Jun fusion protein at a concentration of 2 ug/20 uL reaction was phosphorylated in an in vitro kinase assay with 200 uM ATP.After 30 minutes incubation at 30°C, phosphorylation was detected by Western blot with phospho-specific c-Jun antibodies.
In 20 mM Tris-HCl, 50 mM NaCl, pH 7.5 (1 mM Dithiothreitol, 2 mM EDTA, 50% glycerol)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq
Jun activation domain binding protein,OTTHUMP00000010036,activator protein 1,enhancer-binding protein AP1,v-jun avian sarcoma virus 17 oncogene homolog,v-jun sarcoma virus 17 oncogene homolog