Human WNT1 (P04628, 343 amino acids) partial recombinant protein expressed in Escherichia coli.
Theoretical MW (kDa):
Escherichia coli expression system
>= 98% by SDS-PAGE and HPLC
< 0.1 ng/ug
The ED50 was determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells. The expected ED50 for this effect is 1.5-2.5 ng/mL in the presence of 200 ng/mL of human BMP-2.
Quality Control Testing:
SDS-PAGE Stained with Coomassie Blue
Store at -20°C on dry atmosphere. After reconstitution with sterilized water, store at -20°C. Aliquot to avoid repeated freezing and thawing.
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region. [provided by RefSeq
Wingless-type MMTV integration site family, member 1 (oncogene INT1)