Human NGF (P01138, 119 amino acids) partial recombinant protein expressed in CHO cells.
Theoretical MW (kDa):
Mammalian cell (CHO) expression system
>= 98% by SDS-PAGE and HPLC
< 0.1 ng/ug
The ED50 was determined using PC12 cell line is 1.2-2 ng/mL and using DRG cell line is 0.8-1.5 ng/mL.
Lyophilized from 30 mM sodium acetate, pH 5.5 (1% HAS)
Store at -20°C on dry atmosphere. After reconstitution with deionized water, it is recommended to add a carrier protein (0.1% HAS or BSA) and store at -20°C. Aliquot to avoid repeated freezing and thawing.
This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq