Human IL1B (P01584, 153 amino acids) partial recombinant protein expressed in Escherichia coli.
Theoretical MW (kDa):
17
Form:
Lyophilized
Preparation Method:
Escherichia coli expression system
Purity:
≥ 98% by SDS-PAGE and HPLC
Endotoxin Level:
< 0.1 ng/ug
Activity:
The ED50 was determined by the dose-dependent stimulation of thymidine uptake by murine C3H/HeJ thymocytes is < 0.1 ng/mL, corresponding to a specific activity of > 1 x 107 units/mg.
Storage Buffer:
No additive
Storage Instruction:
Store at -20°C on dry atmosphere. After reconstitution with deionized water, store at 4°C for 1 week or -20°C for longer. Aliquot to avoid repeated freezing and thawing.
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq