ABO (Human) Recombinant Protein
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Specification
Product Description
Human ABO (NP_065202, 54 a.a. - 354 a.a. ) full-length recombinant protein with His tag expressed in Escherichia coli.
Sequence
MGSSHHHHHHSSGLVPRGSHMAVREPDHLQRVSLPRMVYPQPKVLTPCRKDVLVVTPWLAPIVWEGTFNIDILNEQFRLQNTTIGLTVFAIKKYVAFLKLFLETAEKHFMVGHRVHYYVFTDQPAAVPRVTLGTGRQLSVLEVRAYKRWQDVSMRRMEMISDFCERRFLSEVDYLVCVDVDMEFRDHVGVEILTPLFGTLHPGFYGSSREAFTYERRPQSQAYIPKDEGDFYYLGGFFGGSVQEVQRLTRACHQAMMVDQANGIEAVWHDESHLNKYLLRHKPTKVLSPEYLWDQQLLGWPAVLRKLRFTAVPKNHQAVRNP
Host
Escherichia coli
Theoretical MW (kDa)
37.4
Form
Liquid
Preparation Method
Escherichia coli expression system
Purification
Conventional Chromatography
Concentration
0.5 mg/mL
Purity
> 85% by SDS-PAGE
Quality Control Testing
Loading 3 ug protein in 15% SDS PAGE.
Storage Buffer
In 20 mM Tris-HCl buffer, 200 mM NaCl, pH 8.0 (2 mM DTT, 20% glycerol).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
SDS-PAGE
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Gene Info — ABO
Entrez GeneID
28Protein Accession#
NP_065202Gene Name
ABO
Gene Alias
A3GALNT, A3GALT1, GTB, NAGAT
Gene Description
ABO blood group (transferase A, alpha 1-3-N-acetylgalactosaminyltransferase; transferase B, alpha 1-3-galactosyltransferase)
Omim ID
110300Gene Ontology
HyperlinkGene Summary
This gene encodes proteins related to the first discovered blood group system, ABO. Which allele is present in an individual determines the blood group. The 'O' blood group is caused by a deletion of guanine-258 near the N-terminus of the protein which results in a frameshift and translation of an almost entirely different protein. Individuals with the A, B, and AB alleles express glycosyltransferase activities that convert the H antigen into the A or B antigen. Other minor alleles have been found for this gene. [provided by RefSeq
Other Designations
ABO glycocyltransferase|ABO glycosyltransferase|B(A) alpha-1,3-galactosyltransferase|alpha 1-3-N-acetylgalactosaminyltransferase|histo-blood group A2 transferase|histo-blood group ABO protein
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Interactome
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Pathway
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Disease
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