TSLP (Human) Recombinant Protein
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Specification
Product Description
Human TSLP (Q969D9, 29 a.a. - 159 a.a.) partial recombinant protein expressed in Escherichia coli.
Sequence
YDFTNCDFEKIKAAYLSTISKDLITYMSGTKSTEFNNTVSCSNRPHCLTEIQSLTFNPTAGCASLAKEMFAMKTKAALAIWCPGYSETQINATQAMKKRRKRKVTTNKCLEQVSQLQGLWRRFNRPLLKQQ
Host
Escherichia coli
Theoretical MW (kDa)
15
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
Ion exchange column and HPLC reverse phase column
Purity
> 90% by SDS-PAGE and HPLC
Endotoxin Level
< 0.1 ng/ug (1 EU/ug)
Activity
The ED50 was determined by the dose-dependent proliferation of human IL-7R-alpha and human TSLP R co-transfected mouse BaF3 cells, was found to be <3.0 ng/mL.
Storage Buffer
Lyophilized from 20 mM PB, 150mM NaCl, pH 7.4
Storage Instruction
Store at -20°C on dry atmosphere for 2 years.
After reconstitution with deionized water, store at 4°C for 1 month or store at -20°C for 6 months.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — TSLP
Entrez GeneID
85480Protein Accession#
Q969D9Gene Name
TSLP
Gene Alias
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Gene Description
thymic stromal lymphopoietin
Omim ID
607003Gene Ontology
HyperlinkGene Summary
This gene encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. Alternative splicing of this gene results in two transcript variants. [provided by RefSeq
Other Designations
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