OSM (Human) Recombinant Protein
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Specification
Product Description
Human OSM (P13725, 26 a.a. - 252 a.a.) partial recombinant protein expressed in Escherichia coli.
Sequence
AAIGSCSKEYRVLLGQLQKQTDLMQDTSRLLDPYIRIQGLDVPKLREHCRERPGAFPSEETLRGLGRRGFLQTLNATLGCVLHRLADLEQRLPKAQDLERSGLNIEDLEKLQMARPNILGLRNNIYCMAQLLDNSDTAEPTKAGRGASQPPTPTPASDAFQRKLEGCRFLHGYHRFMHSVGRVFSKWGESPNRSRRHSPHQALRKGVRRTRPSRKGKRLMTRGQLPR
Host
Escherichia coli
Theoretical MW (kDa)
26
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
Ion exchange column and HPLC reverse phase column
Purity
> 90% by SDS-PAGE and HPLC
Endotoxin Level
< 0.1 ng/ug (1 EU/ug)
Activity
The ED50 was determined by the dose-dependent stimulation of the proliferation of f human TF-1 cells is <= 3 ng/mL, corresponding to a specific activity of >= 2 x 105 units/mg.
Storage Buffer
Lyophilized from PBS, pH 7.0
Storage Instruction
Store at -20°C on dry atmosphere for 2 years.
After reconstitution with deionized water, store at 4°C for 1 month or store at -20°C for 6 months.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — OSM
Entrez GeneID
5008Protein Accession#
P13725Gene Name
OSM
Gene Alias
MGC20461
Gene Description
oncostatin M
Omim ID
165095Gene Ontology
HyperlinkGene Summary
Oncostatin M is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. This gene encodes a growth regulator which inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. [provided by RefSeq
Other Designations
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