CCL3 (Human) Recombinant Protein
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Specification
Product Description
Human CCL3 (P10147, 24 a.a. - 92 a.a.) partial recombinant protein expressed in Escherichia coli.
Sequence
SLAADTPTACCFSYTSRQIPQNFIADYFETSSQCSKPGVIFLTKRSRQVCADPSEEWVQKYVSDLELSA
Host
Escherichia coli
Theoretical MW (kDa)
8
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
Ion exchange column and HPLC reverse phase column
Purity
> 90% by SDS-PAGE and HPLC
Endotoxin Level
< 0.1 ng/ug (1 EU/ug)
Activity
Determined by the ability to chemoattract human monocytes using a concentration range of 1.0-10.0 ng/mL.
Storage Buffer
Lyophilized from PBS, pH 7.5
Storage Instruction
Store at -20°C on dry atmosphere for 2 years.
After reconstitution with deionized water, store at 4°C for 1 month or store at -20°C for 6 months.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — CCL3
Entrez GeneID
6348Protein Accession#
P10147Gene Name
CCL3
Gene Alias
G0S19-1, LD78ALPHA, MIP-1-alpha, MIP1A, SCYA3
Gene Description
chemokine (C-C motif) ligand 3
Gene Ontology
HyperlinkGene Summary
Macrophage inflammatory protein-1 is a so-called monokine that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes (Wolpe et al., 1988 [PubMed 3279154]). Sherry et al. (1988) [PubMed 3058856] demonstrated 2 protein components of MIP1, called by them alpha and beta.[supplied by OMIM
Other Designations
LD78 alpha beta|small inducible cytokine A3 (homologous to mouse Mip-1a)
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