FGF7 (Human) Recombinant Protein
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Specification
Product Description
Human FGF7 (P21781, 32 a.a. - 194 a.a.) partial recombinant protein expressed in Escherichia coli.
Sequence
CNDMTPEQMATNVNCSSPERHTRSYDYMEGGDIRVRRLFCRTQWYLRIDKRGKVKGTQEMKNNYNIMEIRTVAVGIVAIKGVESEFYLAMNKEGKLYAKKECNEDCNFKELILENHYNTYASAKWTHNGGEMFVALNQKGIPVRGKKTKKEQKTAHFLPMAIT
Host
Escherichia coli
Theoretical MW (kDa)
19
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
Ion exchange column and HPLC reverse phase column
Purity
> 90% by SDS-PAGE and HPLC
Endotoxin Level
< 0.1 ng/ug (1 EU/ug)
Activity
The ED50 was determined by the dose-dependent proliferation of BAF3 cells was found to be less than 10 ng/mL.
Storage Buffer
Lyophilized from 1M NaCl, 20mM PB, pH 8.0
Storage Instruction
Store at -20°C on dry atmosphere for 2 years.
After reconstitution with deionized water, store at 4°C for 1 month or store at -20°C for 6 months.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — FGF7
Entrez GeneID
2252Protein Accession#
P21781Gene Name
FGF7
Gene Alias
HBGF-7, KGF
Gene Description
fibroblast growth factor 7 (keratinocyte growth factor)
Omim ID
148180Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis. [provided by RefSeq
Other Designations
fibroblast growth factor 7|heparin-binding growth factor 7|keratinocyte growth factor
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