Human IL4 (P05112, His 25 - Ser 153) partial recombinant protein expressed in Escherichia coli.
Host:
Escherichia coli
Theoretical MW (kDa):
15
Form:
Lyophilized
Preparation Method:
Escherichia coli expression system
Purification:
Ion exchange column and HPLC reverse phase column
Purity:
> 90% by SDS-PAGE and HPLC
Endotoxin Level:
< 0.1 ng/ug (1 EU/ug)
Activity:
The ED50 was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is <= 0.1 ng/mL, corresponding to a specific activity of >1 x 107 units/mg.
Storage Buffer:
Lyophilized from 2.5% glycine, 0.5% sucrose, 0.01% Tween 80, 5 mM Glutamic acid, pH 4.5
Storage Instruction:
Store at -20°C on dry atmosphere for 2 years. After reconstitution with deionized water, store at 4°C for 1 month or store at -20°C for 6 months. Aliquot to avoid repeated freezing and thawing.
The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq