IL3 (Human) Recombinant Protein
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Specification
Product Description
Human IL3 (P08700, 20 a.a. - 152 a.a.) partial recombinant protein expressed in Escherichia coli.
Sequence
APMTQTTPLKTSWVNCSNMIDEIITHLKQPPLPLLDFNNLNGEDQDILMENNLRRPNLEAFNRAVKSLQNASAIESILKNLLPCLPLATAAPTRHPIHIKDGDWNEFRRKLTFYLKTLENAQAQQTTLSLAIF
Host
Escherichia coli
Theoretical MW (kDa)
15
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
Ion exchange column and HPLC reverse phase column
Purity
> 90% by SDS-PAGE and HPLC
Endotoxin Level
< 0.1 ng/ug (1 EU/ug)
Activity
The ED50 was determined by the dose-dependent proliferation of Human TF-1 cells was found less than 0.1 ng/mL.
Storage Buffer
Lyophilized from PBS, pH 7.0
Storage Instruction
Store at -20°C on dry atmosphere for 2 years.
After reconstitution with deionized water, store at 4°C for 1 month or store at -20°C for 6 months.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — IL3
Entrez GeneID
3562Protein Accession#
P08700Gene Name
IL3
Gene Alias
IL-3, MCGF, MGC79398, MGC79399, MULTI-CSF
Gene Description
interleukin 3 (colony-stimulating factor, multiple)
Omim ID
147740Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq
Other Designations
P-cell stimulating factor|hematopoietic growth factor|interleukin 3|mast-cell growth factor|multilineage-colony-stimulating factor
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