CCL14 (Human) Recombinant Protein
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Specification
Product Description
Human CCL14 (Q16627, 22 a.a. - 93 a.a.) partial recombinant protein expressed in Escherichia coli.
Sequence
KTESSSRGPYHPSECCFTYTTYKIPRQRIMDYYETNSQCSKPGIVFITKRGHSVCTNPSDKWVQDYIKDMKEN
Host
Escherichia coli
Theoretical MW (kDa)
9
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
Ion exchange column and HPLC reverse phase column
Purity
> 90% by SDS-PAGE and HPLC
Endotoxin Level
< 0.1 ng/ug (1 EU/ug)
Activity
Determined by the ability to chemoattract human monocytes using a concentration range of 2.0-40.0 ng/mL.
Storage Buffer
Lyophilized from 20 mM PB, 100 mM NaCl, pH 7.5
Storage Instruction
Store at -20°C on dry atmosphere for 2 years.
After reconstitution with deionized water, store at 4°C for 1 month or store at -20°C for 6 months.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — CCL14
Entrez GeneID
6358Protein Accession#
Q16627Gene Name
CCL14
Gene Alias
CC-1, CC-3, CKb1, HCC-1, HCC-3, MCIF, NCC-2, NCC2, SCYA14, SCYL2, SY14
Gene Description
chemokine (C-C motif) ligand 14
Omim ID
601392Gene Ontology
HyperlinkGene Summary
This gene, CCL14, is one of several CC cytokine genes clustered on 17q11.2. The CC cytokines are secreted proteins characterized by two adjacent cysteines. The cytokine encoded by this gene induces changes in intracellular calcium concentration and enzyme release in monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Read-through transcripts are also expressed that include exons from the upstream cytokine gene CCL15, and are represented as GeneID: 348249. [provided by RefSeq
Other Designations
OTTHUMP00000176860|chemokine CC-1|chemokine CC-3|small inducible cytokine subfamily A (Cys-Cys), member 14
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