FGF9 (Human) Recombinant Protein
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Specification
Product Description
Human FGF9 (P31371, 1 a.a. - 208 a.a.) full-length recombinant protein. expressed in Escherichia coli.
Sequence
MAPLGEVGNYFGVQDAVPFGNVPVLPVDSPVLLSDHLGQSEAGGLPRGPAVTDLDHLKGILRRRQLYCRTGFHLEIFPNGTIQGTRKDHSRFGILEFISIAVGLVSIRGVDSGLYLGMNEKGELYGSEKLTQECVFREQFEENWYNTYSSNLYKHVDTGRRYYVALNKDGTPREGTRTKRHQKFTHFLPRPVDPDKVPELYKDILSQS
Host
Escherichia coli
Theoretical MW (kDa)
23
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
Ion exchange column and HPLC reverse phase column
Purity
> 90% by SDS-PAGE and HPLC
Endotoxin Level
< 0.1 ng/ug (1 EU/ug)
Activity
The ED50 was determined by the dose-dependent proliferationof BaF3 cells expressing FGF receptors and was found to be in the range of 0.6 ng/mL.
Storage Buffer
Lyophilized from PBS
Storage Instruction
Store at -20°C on dry atmosphere for 2 years.
After reconstitution with deionized water, store at 4°C for 1 month or store at -20°C for 6 months.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — FGF9
Entrez GeneID
2254Protein Accession#
P31371Gene Name
FGF9
Gene Alias
GAF, HBFG-9, MGC119914, MGC119915
Gene Description
fibroblast growth factor 9 (glia-activating factor)
Omim ID
600921Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homolog of this gene was found to be dependent on Sonic hedgehog (Shh) signaling. Mice lacking the homolog gene displayed a male-to-female sex reversal phenotype, which suggested a role in testicular embryogenesis. [provided by RefSeq
Other Designations
OTTHUMP00000018804|fibroblast growth factor 9|glia-activating factor
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