EPO (Human) Recombinant Protein
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Specification
Product Description
Human EPO (P01588, 28 a.a. - 193 a.a.) partial recombinant protein expressed in CHO cells.
Sequence
APPRLICDSRVLERYLLEAKEAENITTGCAEHCSLNENITVPDTKVNFYAWKRMEVGQQAVEVWQGLALLSEAVLRGQALLVNSSQPWEPLQLHVDKAVSGLRSLTTLLRALGAQKEAISPPDAASAAPLRTITADTFRKLFRVYSNFLRGKLKLYTGEACRTGDR
Host
Mammals
Theoretical MW (kDa)
35
Form
Lyophilized
Preparation Method
Mammalian cell (CHO) expression system
Purification
Ion exchange column and HPLC reverse phase column
Purity
> 90% by SDS-PAGE and HPLC
Endotoxin Level
< 0.1 ng/ug (1 EU/ug)
Activity
Activity was determined by the dose-dependent proliferation assay using a factor-dependent human erythroleukemic cell line TF-1 and was found to be 3.8 x 105 IU/mg.
Storage Buffer
Lyophilized from PBS, pH 7
Storage Instruction
Store at -20°C on dry atmosphere for 2 years.
After reconstitution with deionized water, store at 4°C for 1 month or store at -20°C for 6 months.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — EPO
Entrez GeneID
2056Protein Accession#
P01588Gene Name
EPO
Gene Alias
EP, MGC138142
Gene Description
erythropoietin
Omim ID
133170Gene Ontology
HyperlinkGene Summary
This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. [provided by RefSeq
Other Designations
epoetin
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Interactome
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Disease
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