DEFB104A (Human) Recombinant Protein
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Specification
Product Description
Human DEFB104A (Q8WTQ1, 23 a.a. - 72 a.a.) partial recombinant protein expressed in Escherichia coli.
Sequence
EFELDRICGYGTARCRKKCRSQEYRIGRCPNTYACCLRKWDESLLNRTKP
Host
Escherichia coli
Theoretical MW (kDa)
6
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
Ion exchange column and HPLC reverse phase column
Purity
> 90% by SDS-PAGE and HPLC
Endotoxin Level
< 0.1 ng/ug (1 EU/ug)
Activity
The ED50 was determined by the ability to chemoattract immature human dendritic cells using a concentration range of 1-50 ng/mL.
Storage Buffer
Lyophilized from 100 mM NaCl, 20 mM PB, pH 7.4
Storage Instruction
Store at -20°C on dry atmosphere for 2 years.
After reconstitution with deionized water, store at 4°C for 1 month or store at -20°C for 6 months.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — DEFB104A
Entrez GeneID
140596Protein Accession#
Q8WTQ1Gene Name
DEFB104A
Gene Alias
BD-4, DEFB-4, DEFB104, DEFB4, MGC118942, MGC118944, MGC118945, hBD-4
Gene Description
defensin, beta 104A
Gene Ontology
HyperlinkGene Summary
Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Defensins are short, processed peptide molecules that are classified by structure into three groups: alpha-defensins, beta-defensins and theta-defensins. All beta-defensin genes are densely clustered in four to five syntenic chromosomal regions. Chromosome 8p23 contains at least two copies of the duplicated beta-defensin cluster. This duplication results in two identical copies of defensin, beta 104, DEFB104A and DEFB104B, in head-to-head orientation. This gene, DEFB104A, represents the more centromeric copy. [provided by RefSeq
Other Designations
defensin, beta 4
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