DEFB1 (Human) Recombinant Protein
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Specification
Product Description
Human DEFB1 (P60022, 33 a.a. - 70 a.a.) partial recombinant protein expressed in Escherichia coli.
Sequence
RSDHYNCVSSGGQCLYSACPIFTKIQGTCYRGKAKCCK
Host
Escherichia coli
Theoretical MW (kDa)
5
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
Ion exchange column and HPLC reverse phase column
Purity
> 90% by SDS-PAGE and HPLC
Endotoxin Level
< 0.1 ng/ug (1 EU/ug)
Activity
The ED50 was determined by the ability to chemoattract CD34+ dendritic cells using a concentration range of 0.1-1.0 ug/mL.
Storage Buffer
Lyophilized from 100 mM NaCl, 20 mM PB, pH 7.4
Storage Instruction
Store at -20°C on dry atmosphere for 2 years.
After reconstitution with deionized water, store at 4°C for 1 month or store at -20°C for 6 months.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — DEFB1
Entrez GeneID
1672Protein Accession#
P60022Gene Name
DEFB1
Gene Alias
BD1, DEFB-1, DEFB101, HBD1, MGC51822
Gene Description
defensin, beta 1
Omim ID
602056Gene Ontology
HyperlinkGene Summary
Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 1, an antimicrobial peptide implicated in the resistance of epithelial surfaces to microbial colonization. This gene maps in close proximity to defensin family member, defensin, alpha 1 and has been implicated in the pathogenesis of cystic fibrosis. [provided by RefSeq
Other Designations
beta-defensin-1
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Interactome
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