CA2 (Human) Recombinant Protein
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Specification
Product Description
Human CA2 (NP_000058, 1 a.a. - 260 a.a.) full-length recombinant protein expressed in Escherichia coli.
Sequence
MSHHWGYGKHNGPEHWHKDFPIAKGERQSPVDIDTHTAKYDPSLKPLSVSYDQATSLRILNNGHAFNVEFDDSQDKAVLKGGPLDGTYRLIQFHFHWGSLDGQGSEHTVDKKKYAAELHLVHWNTKYGDFGKAVQQPDGLAVLGIFLKVGSAKPGLQKVVDVLDSIKTKGKSADFTNFDPRGLLPESLDYWTYPGSLTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGEGEPEELMVDNWRPAQPLKNRQIKASFK
Host
Escherichia coli
Theoretical MW (kDa)
29.2
Form
Liquid
Preparation Method
Escherichia coli expression system
Purification
Conventional Chromatography
Concentration
1 mg/mL
Purity
> 95% by SDS-PAGE
Activity
The specific activity is 50-70 nmol/min/ug and was determined by measuring the increase in the amount of p-nitrophenol by the esterase activity. The specific activity is defined as the amount of p-nitrophenol that can reduce 1 ug of the enzyme at 25°C for 1 minute.
Quality Control Testing
Loading 3 ug protein in 15% SDS-PAGE
Storage Buffer
In 20 mM Tris-HCl, 50 mM NaCl, pH 8.0 (1 mM DTT, 10% glycerol).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — CA2
Entrez GeneID
760Protein Accession#
NP_000058Gene Name
CA2
Gene Alias
CA-II, CAII, Car2
Gene Description
carbonic anhydrase II
Gene Ontology
HyperlinkGene Summary
CA2 is one of several (at least 7) isozymes of carbonic anhydrase. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. [provided by RefSeq
Other Designations
carbonate dehydratase II|carbonic anhydrase B|carbonic dehydratase
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