PRDX2 (Human) Recombinant Protein
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Specification
Product Description
Human PRDX2 (NP_005800, 1 a.a. - 198 a.a.) full-length recombinant protein expressed in Escherichia coli.
Sequence
MASGNARIGKPAPDFKATAVVDGAFKEVKLSDYKGKYVVLFFYPLDFTFVCPTEIIAFSNRAEDFRKLGCEVLGVSVDSQFTHLAWINTPRKEGGLGPLNIPLLADVTRRLSEDYGVLKTDEGIAYRGLFIIDGKGVLRQITVNDLPVGRSVDEALRLVQAFQYTDEHGEVCPAGWKPGSDTIKPNVDDSKEYFSKHN
Host
Escherichia coli
Theoretical MW (kDa)
21.8
Form
Liquid
Preparation Method
Escherichia coli expression system
Purification
Conventional Chromatography
Concentration
1 mg/mL
Purity
> 90% by SDS-PAGE
Activity
Specific activity: approximately 200-230 pmole/min/ug. Enzymatic activity was confirmed by measuring the remaining peroxide after incubation of PRDX2 and peroxide for 20 min at room temperature. Specific activity is defined as the amount of hydroperoxide
Quality Control Testing
Loading 3 ug protein in 15% SDS-PAGE
Storage Buffer
In 20 mM Tris-HCl buffer, pH 8.0 (10% glycerol).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — PRDX2
Entrez GeneID
7001Protein Accession#
NP_005800Gene Name
PRDX2
Gene Alias
MGC4104, NKEFB, PRP, PRX2, PRXII, TDPX1, TSA
Gene Description
peroxiredoxin 2
Omim ID
600538Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. The crystal structure of this protein has been resolved to 2.7 angstroms. Transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq
Other Designations
natural killer-enhancing factor B|thiol-specific antioxidant 1|thioredoxin peroxidase 1|thioredoxin-dependent peroxide reductase 1|torin
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