GLO1 (Human) Recombinant Protein
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Specification
Product Description
Human GLO1 (NP_006699, 1 a.a. - 184 a.a.) full-length recombinant protein expressed in Escherichia coli.
Sequence
MAEPQPPSGGLTDEAALSCCSDADPSTKDFLLQQTMLRVKDPKKSLDFYTRVLGMTLIQKCDFPIMKFSLYFLAYEDKNDIPKEKDEKIAWALSRKATLELTHNWGTEDDETQSYHNGNSDPRGFGHIGIAVPDVYSACKRFEELGVKFVKKPDDGKMKGLAFIQDPDGYWIEILNPNKMATLM
Host
Escherichia coli
Theoretical MW (kDa)
20.7
Form
Liquid
Preparation Method
Escherichia coli expression system
Purification
Conventional Chromatography
Concentration
1 mg/mL
Purity
> 90% by SDS-PAGE
Activity
Specific activity: >0.4 units/mg (please enquire for specific batch value). One unit will form 1.0 umol of S-lactoylglutathione from methylglyoxal and reduced glutathione per minute at pH 7.5, at 25°C. Specific activity was expressed in units/mg of
Quality Control Testing
Loading 3 ug protein in 15% SDS-PAGE
Storage Buffer
In 20 mM Tris-HCl buffer, pH 8.0 (1 mM DTT, 10% glycerol).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — GLO1
Entrez GeneID
2739Protein Accession#
NP_006699Gene Name
GLO1
Gene Alias
GLOD1, GLYI
Gene Description
glyoxalase I
Gene Ontology
HyperlinkGene Summary
The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq
Other Designations
OTTHUMP00000016339|glyoxalase domain containing 1|lactoyl glutathione lyase|lactoylglutathione lyase
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Interactome
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