VEGFA (Human) Recombinant Protein
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Specification
Product Description
Human VEGFA (NP_001020541, 207 a.a. - 327 a.a.) partial recombinant protein with His tag expressed in Escherichia coli.
Sequence
MGSSHHHHHHSSGLVPRGSHMAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQEKCDKPRR
Host
Escherichia coli
Theoretical MW (kDa)
16.3
Form
Liquid
Preparation Method
Escherichia coli expression system
Purification
Conventional Chromatography
Concentration
0.5 mg/mL
Purity
> 90% by SDS-PAGE
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Activity
The ED50 for this effect is < 4.2 ng/mL. Measured in a cell proliferation assay using NIH-3T3 cell.
Quality Control Testing
Loading 3 ug protein in 15% SDS-PAGE
Storage Buffer
In 20 mM Tris, pH 8.0 (10% glycerol).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — VEGFA
Entrez GeneID
7422Protein Accession#
NP_001020541Gene Name
VEGFA
Gene Alias
MGC70609, VEGF, VEGF-A, VPF
Gene Description
vascular endothelial growth factor A
Gene Ontology
HyperlinkGene Summary
This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq
Other Designations
vascular endothelial growth factor isoform VEGF165|vascular permeability factor
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