DPP4 (Human) Recombinant Protein
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Specification
Product Description
Human DPP4 (NP_001926, 39 a.a. - 766 a.a.) partial recombinant protein with His tag expressed in Hi-5 cell.
Sequence
ADP-SRKTYTLTDYLKNTYRLKLYSLRWISDHEYLYKQENNILVFNAEYGNSSVFLENSTFDEFGHSINDYSISPDGQFILLEYNYVKQWRHSYTASYDIYDLNKRQLITE ERIPNNTQWVTWSPVGHKLAYVWNNDIYVKIEPNLPSYRITWTGKEDIIYNGITDWVYEEEVFSAYSALWWSPNGTFLAYAQFNDTEVPL IEYSFYSDESLQYPKTVRVPYPKAGAVNPTVKFFVVNTDSLSSVTNATSIQITAPASMLIGDHYLCDVTWATQERISLQWLRRIQNYSVM DICDYDESSG RWNCLVARQHIEMSTTGWVGRFRPSEPHFTLDGNSFYKIISNEEGYRHICYFQIDKKDCTFITKGTWEVIGIEALTSDYLYYISNEYKGM PGGRNLYKIQ LSDYTKVTCLSCELNPERCQYYSVSFSKEAKYYQLRCSGPGLPLYTLHSSVNDKGLRVLEDNSALDKMLQNVQMPSKKLDFIILNETKFWYQMILPPHFD KSKKYPLLLDVYAGPCSQKADTVFRLNWATYLASTENIIVASFDGRGSGYQGDKIMHAINRRLGTFEVEDQIEAARQFSKMGFVDNKRIAIWGWSYGGYV TSMVLGSGSGVFKCGIAVAPVSRWEYYDSVYTERYMGLPTPEDNLDHYRNSTVMSRAENFKQVEYLLIHGTADDNVHFQQSAQISKALVDVGVDFQAMWY TDEDHGIASSTAHQHIYTHMSHFIKQCFSL PHHHHHH
Host
insect
Theoretical MW (kDa)
86.40000000000001
Form
Liquid
Preparation Method
Insect cell (Hi-5) expression system
Purification
Conventional Chromatography
Concentration
0.5 mg/mL
Purity
> 95% by SDS-PAGE
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Activity
Approximately > 200 Unit/mg. One unit will hydrolyze 1.0 umole of p-nitroaniline per minute at pH 8.0 at 37°C using Gly-Pro- p-Nitroaniline as a substrate.
Quality Control Testing
Loading 3 ug protein in 15% SDS-PAGE
Storage Buffer
In 20 mM Tris-HCl, 100 mM NaCl, 1 mM EDTA, pH 8.0 (10% glycerol).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — DPP4
Entrez GeneID
1803Protein Accession#
NP_001926Gene Name
DPP4
Gene Alias
ADABP, ADCP2, CD26, DPPIV, TP103
Gene Description
dipeptidyl-peptidase 4
Omim ID
102720Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. [provided by RefSeq
Other Designations
T-cell activation antigen CD26|adenosine deaminase complexing protein 2|dipeptidylpeptidase 4|dipeptidylpeptidase IV|dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2)
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Interactome
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Disease
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Publication Reference
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Dipeptidyl peptidase-4 is highly expressed in bronchial epithelial cells of untreated asthma and it increases cell proliferation along with fibronectin production in airway constitutive cells.
Shiobara T, Chibana K, Watanabe T, Arai R, Horigane Y, Nakamura Y, Hayashi Y, Shimizu Y, Takemasa A, Ishii Y.
Respiratory Research 2016 Mar; 17:28.
Application：Func, Human, HFL-1, BSMC.
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Dipeptidyl peptidase-4 is highly expressed in bronchial epithelial cells of untreated asthma and it increases cell proliferation along with fibronectin production in airway constitutive cells.
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