PPID (Human) Recombinant Protein
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Specification
Product Description
Human PPID (NP_005029, 1 a.a. - 370 a.a.) full-length recombinant protein with His tag expressed in Escherichia coli.
Sequence
MGSSHHHHHHSSGLVPRGSHMSHPSPQAKPSNPSNPRVFFDVDIGGERVGRIVLELFADIVPKTAENFRALCTGEKGIGHTTGKPLHFKGCPFHRIIKKFMIQGGDFSNQNGTGGESIYGEKFEDENFHYKHDREGLLSMANAGRNTNGSQFFITTVPTPHLDGKHVVFGQVIKGIGVARILENVEVKGEKPAKLCVIAECGELKEGDDGGIFPKDGSGDSHPDFPEDADIDLKDVDKILLITEDLKNIGNTFFKSQNWEMAIKKYAEVLRYVDSSKAVIETADRAKLQPIALSCVLNIGACKLKMSNWQGAIDSCLEALELDPSNTKALYRRAQGWQGLKEYDQALADLKKAQGIAPEDKAIQAELLKVKQKIKAQKDKEKAVYAKMFA
Host
Escherichia coli
Theoretical MW (kDa)
42.9
Form
Liquid
Preparation Method
Escherichia coli expression system
Purification
Conventional Chromatography
Concentration
1 mg/mL
Purity
> 95% by SDS-PAGE
Activity
Specific activity is > 210 nmoles/min/ug, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25°C in Tris-HCl pH 8.0 using chymotrypsin.
Quality Control Testing
Loading 3 ug protein in 15% SDS-PAGE
Storage Buffer
In PBS, pH 7.4 (10% glycerol).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — PPID
Entrez GeneID
5481Protein Accession#
NP_005029Gene Name
PPID
Gene Alias
CYP-40, CYPD, MGC33096
Gene Description
peptidylprolyl isomerase D
Omim ID
601753Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein has been shown to possess PPIase activity and, similar to other family members, can bind to the immunosuppressant cyclosporin A. [provided by RefSeq
Other Designations
40 kDa peptidyl-prolyl cis-trans isomerase D|PPIase|cyclophilin 40|cyclophilin D|cyclophilin-related protein|rotamase
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Interactome
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Pathway
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