IL3 (Human) Recombinant Protein
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Specification
Product Description
Human IL3 (AAC08706, 20 aa. - 152 a.a.) partial recombinant protein with His tag expressed in Escherichia coli.
Sequence
MGSSHHHHHHSSGLVPRGSHMAPMTQTTSLKTSWVNCSNMIDEIITHLKQPPLPLLDFNNLNGEDQDILMENNLRRPNLEAFNRAVKSLQNASAIESILKNLLPCLPLATAAPTRHPIHIKDGDWNEFRRKLTFYLKTLENAQAQQTTLSLAIF
Host
Escherichia coli
Theoretical MW (kDa)
17.3
Form
Liquid
Preparation Method
Escherichia coli expression system
Purification
Conventional Chromatography
Concentration
1 mg/mL
Purity
> 90% by SDS-PAGE
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Activity
The ED50 for this effect is <0.53 ng/mL. Measured in a cell proliferation assay using TF1 human erythroleukemic cells.
Quality Control Testing
Loading 3 ug protein in 15% SDS-PAGE
Storage Buffer
In 20 mM Tris-HCl buffer, pH 8.0 (10% glycerol, 0.2 mM PMSF).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — IL3
Entrez GeneID
3562Protein Accession#
AAC08706Gene Name
IL3
Gene Alias
IL-3, MCGF, MGC79398, MGC79399, MULTI-CSF
Gene Description
interleukin 3 (colony-stimulating factor, multiple)
Omim ID
147740Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq
Other Designations
P-cell stimulating factor|hematopoietic growth factor|interleukin 3|mast-cell growth factor|multilineage-colony-stimulating factor
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