< 1.0 EU per 1 microgram of protein (determined by LAL method)
Activity:
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is < 0.16 ng/mL.
Quality Control Testing:
Loading 3 ug protein in 15% SDS-PAGE
Storage Buffer:
In PBS, pH 7.4.
Storage Instruction:
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Aliquot to avoid repeated freezing and thawing.
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq
Other Designations:
APC1 protein,OTTHUMP00000029281,OTTHUMP00000037669,TNF superfamily, member 2,TNF, macrophage-derived,TNF, monocyte-derived,cachectin,tumor necrosis factor alpha