Mouse monoclonal antibody raised against recombinant ACAN.
Recombinant protein correspoding to amino acids ARGSVIL at N-terminus of human ACAN.
The antibody reacts with the N-terminal sequence ARGSVIL of ACAN fragments produced by proteolysis at the ACANase site within the interglobular domain of ACAN (ACAN-IGD). It does not react to a significant extent with the sequence ARGSVIL contained within uncleaved polypeptide chains. ARGSVIL-Peptides produced from recombinant ACAN-IGD in the presence of ACANases bind strongly to the antibody both in Western Blots (see aaplication) and in ELISA. ARGSVIL-fragments derived from native ACAN require prior deglycosylation for antibody binding.
Western blot (1:2000) The optimal working dilution should be determined by the end user.
In 20 mM Na-phosphate, 150 mM NaCl, pH 7.5 (0.01% thimerosal)
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
This product contains thimerosal: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blot of recombinant ACAN-IGD and ACAN (ARGSVIL) monoclonal antibody, clone N38 (Cat # MAB9999 ). Lane 1 : 100 ng uncleaved ACAN-IGD. Lane 2 : 100 ng ARGSVIL-peptide. Lane 3 : 25 ng ARGSVIL-peptide. The apparent Mr of uncleaved ACAN-IGD in SDS-PAGE is 22 kDa.
This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene. [provided by RefSeq