The antibody reacts with extracellular (N-terminal) domain of standard CD44, a 80-95 kDa transmembrane glycoprotein (hyaladherin family)present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells,etc.); it is negative on platelets and hepatocytes.
Flow cytometry (20 ul reagent / 100 ul of whole blood or 106 cells) The optimal working dilution should be determined by the end user.
In PBS (0.09% sodium azide, 0.2% (w/v) BSA)
Store in the dark at 4°C. Avoid prolonged exposure to light. Do not freeze.
Western blot analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using CD44 monoclonal antibody, clone MEM-263 (FITC) (Cat # MAB9988 ). Lane 1: lysate of human PBL. Lane 2: lysate of canine PBL. Lane 3: lysate of porcine PBL.