CD44 monoclonal antibody, clone MEM-263 (FITC)
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More Files
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Western Blot (Cell lysate)
Western blot analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using CD44 monoclonal antibody, clone MEM-263 (FITC) (Cat # MAB9988 ).
Lane 1: lysate of human PBL.
Lane 2: lysate of canine PBL.
Lane 3: lysate of porcine PBL.
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Specification
Product Description
Mouse monoclonal antibody raised against CD44.
Immunogen
COS-7 cells (African Green Monkey)
Host
Mouse
Reactivity
Dog, Human, Monkey, Pig
Specificity
The antibody reacts with extracellular (N-terminal) domain of standard CD44, a 80-95 kDa transmembrane glycoprotein (hyaladherin family)present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells,etc.); it is negative on platelets and hepatocytes.
Form
Liquid
Conjugation
FITC
Isotype
IgG1
Recommend Usage
Flow cytometry (20 ul reagent / 100 ul of whole blood or 106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide, 0.2% (w/v) BSA)
Storage Instruction
Store in the dark at 4°C. Avoid prolonged exposure to light. Do not freeze.
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Applications
Western Blot (Cell lysate)
Western blot analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using CD44 monoclonal antibody, clone MEM-263 (FITC) (Cat # MAB9988 ).
Lane 1: lysate of human PBL.
Lane 2: lysate of canine PBL.
Lane 3: lysate of porcine PBL.Flow Cytometry
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