SPDEF monoclonal antibody, clone 3
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HeLa whole cell lystae with SPDEF monoclonal antibody, clone 3 (Cat # MAB9916) at 1:500 dilution.
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant SPDEF.
Immunogen
Recombinant protein corresponding to full length human SPDEF.
Host
Mouse
Reactivity
Human
Specificity
It can expression in HeLa whole cell lysate.
Form
Liquid
Purification
Affinity purification
Isotype
IgG1
Recommend Usage
Western blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In Citrate-Tris-HCl buffer, pH 7.0 (0.02% Proclin 300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western blot analysis of HeLa whole cell lystae with SPDEF monoclonal antibody, clone 3 (Cat # MAB9916) at 1:500 dilution.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SPDEF
Entrez GeneID
25803GeneBank Accession#
NM_012391.1Gene Name
SPDEF
Gene Alias
PDEF, RP11-375E1__A.3, bA375E1.3
Gene Description
SAM pointed domain containing ets transcription factor
Omim ID
608144Gene Ontology
HyperlinkGene Summary
PDEF is an ETS transcription factor expressed in prostate epithelial cells. It acts as an androgen-independent transactivator of PSA (MIM 176820) expression.[supplied by OMIM
Other Designations
OTTHUMP00000016234|prostate epithelium-specific Ets transcription factor
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Interactome
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Publication Reference
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Upregulation of SPDEF is associated with poor prognosis in prostate cancer.
Meiners J, Schulz K, Möller K, Höflmayer D, Burdelski C, Hube-Magg C, Simon R, Göbel C, Hinsch A, Reiswich V, Weidemann S, Izbicki JR, Sauter G, Jacobsen F, Möller-Koop C, Mandelkow T, Blessin NC, Lutz F, Viehweger F, Lennartz M, Fraune C, Heinzer H, Minner S, Bonk S, Huland H, Graefen M, Schlomm T, Büscheck F.
Oncology Letters 2019 Nov; 18(5):5107.
Application：IHC-P, Human, Human tissue microarray, Prostate cancer.
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Upregulation of SPDEF is associated with poor prognosis in prostate cancer.
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