Rat monoclonal antibody raised against MHC Class II.
Activated C57BL/6 mouse splenocytes.
The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.
Flow Cytometry (4 ug/mL) The optimal working dilution should be determined by the end user.
In PBS, pH 7.4 (0.09% sodium azide)
Store in the dark at 4°C. Avoid prolonged exposure to light. Do not freeze.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.