Mouse monoclonal antibody raised against synthetic peptide of His tag.
A synthetic peptide (conjugated with KLH) corresponding to His tag.
Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags).
Protein A purification
Western Blot (1:1000-1:3000) Immunohistochemistry (1:500-1:2000) The optimal working dilution should be determined by the end user.
In 10 mM PBS, pH 7.2 (0.05% sodium azide)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2). Blotting with His tag monoclonal antibody, clone HIS.H8 (Cat # MAB9752).
Immunofluorescence staining of HEK293 cells. A: untransfected control. B: His tag monoclonal antibody, clone HIS.H8 (Cat # MAB9752) (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue).