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Last updated: 2017/8/20
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His tag monoclonal antibody, clone HIS.H8

  • Catalog # : MAB9752
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic peptide of His tag.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to His tag.
  • Host:
  • Mouse
  • Specificity:
  • Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags).
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Concentration:
  • 1 mg/mL
  • Isotype:
  • IgG2b
  • Recommend Usage:
  • Western Blot (1:1000-1:3000)
    Immunohistochemistry (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 10 mM PBS, pH 7.2 (0.05% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2). Blotting with His tag monoclonal antibody, clone HIS.H8 (Cat # MAB9752).
  • Immunohistochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence staining of HEK293 cells.
    A: untransfected control.
    B: His tag monoclonal antibody, clone HIS.H8 (Cat # MAB9752) (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue).
  • Immunoprecipitation
  • ELISA
  • Dot Blot
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry
  • Immunoprecipitation
  • ELISA
  • Dot Blot
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