HA tag monoclonal antibody, clone HA.C5
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis probed against HEK293 cells transfected with HA-tagged protein vector; untransfected (1) and transfected (2). Blotting with HA tag monoclonal antibody, clone HA.C5 (Cat # MAB9751) at 1:1000 dilution.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of HA tag.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to HA tag.
Host
Mouse
Specificity
Recognizes the N-terminal, C-terminal or internal HA- tagged fusion proteins.
Form
Liquid
Purification
Protein A purification
Concentration
1 mg/mL
Isotype
IgG3
Recommend Usage
Western Blot (1:1000-1:3000)
Immunohistochemistry (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS, pH 7.2 (0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis probed against HEK293 cells transfected with HA-tagged protein vector; untransfected (1) and transfected (2). Blotting with HA tag monoclonal antibody, clone HA.C5 (Cat # MAB9751) at 1:1000 dilution.Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Dot Blot
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