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Last updated: 2016/12/4
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GFP tag monoclonal antibody, clone GF28R

  • Catalog # : MAB9749
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic peptide of GFP tag.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to N-terminus of jellyfish GBP tag.
  • Host:
  • Mouse
  • Reactivity:
  • Jellyfishes
  • Specificity:
  • Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP.
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Concentration:
  • 1 mg/mL
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Western Blot (1:1000-1:3000)
    Immunohistochemistry (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 10 mM PBS, pH 7.2 (0.05% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis probed against HEK293 cells transfected with GFP-tagged protein vector: untransfected control (1), 1 ug (2) and 10 ug (3) of cell lysates used. Blotting with GFP tag monoclonal antibody, clone GF28R (Cat # MAB9749) at 1:1000 dilution.
  • Immunohistochemistry
  • Immunoprecipitation
  • ELISA
  • Dot Blot
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry
  • Immunoprecipitation
  • ELISA
  • Dot Blot
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