GFP tag monoclonal antibody, clone GF28R
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of GFP tag.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of jellyfish GBP tag.
Host
Mouse
Reactivity
Jellyfishes
Specificity
Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP.
Form
Liquid
Purification
Protein A purification
Concentration
1 mg/mL
Isotype
IgG1
Recommend Usage
Western Blot (1:1000-1:3000)
Immunohistochemistry (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS, pH 7.2 (0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis probed against HEK293 cells transfected with GFP-tagged protein vector: untransfected control (1), 1 ug (2) and 10 ug (3) of cell lysates used. Blotting with GFP tag monoclonal antibody, clone GF28R (Cat # MAB9749) at 1:1000 dilution.Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Dot Blot
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