GAPDH monoclonal antibody, clone GA1R
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant GAPDH.
Immunogen
Recombinant protein corrsponding to GAPDH.
Host
Mouse
Reactivity
Chicken, Hamster, Human, Mouse, Rabbit, Rat
Specificity
Recognizes native and denatured forms of GAPDH (~37 KDa).
Form
Liquid
Purification
Protein A purification
Concentration
1 mg/mL
Isotype
IgG1
Recommend Usage
Western Blot (1:1000-1:10000)
Immunohistochemistry (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS, pH 7.2 (0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of 5 ug/lane tissue lysates from human (1), mouse (2), rat (3), rabbit (4), chicken (5), and hamster (6). Blotting with GAPDH monoclonal antibody, clone GA1R (Cat # MAB9748) at 1:2000 dilution.Western Blot
Western blot from BL-21 bacteria (1), Sf9 insect (2), and Saccharomyces cerevisiae (3) with GAPDH monoclonal antibody, clone GA1R (Cat # MAB9748).Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
Dot Blot
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