DYKDDDDK tag monoclonal antibody, clone FG4R
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis probed against HEK293 cells transfected with DYKDDDDK-tagged protein vector; untransfected (1) and transfected (2). Blotting with DYKDDDDK tag monoclonal antibody, clone FG4R (Cat # MAB9744) at 1:1000 dilution.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of DYKDDDDK tag.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to DYKDDDDK tag.
Host
Mouse
Specificity
Recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins.
Form
Liquid
Purification
Protein A purification
Concentration
1 mg/mL
Isotype
IgG2b
Recommend Usage
Western Blot (1:1000-1:3000)
Immunohistochemistry (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS, pH 7.2 (0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis probed against HEK293 cells transfected with DYKDDDDK-tagged protein vector; untransfected (1) and transfected (2). Blotting with DYKDDDDK tag monoclonal antibody, clone FG4R (Cat # MAB9744) at 1:1000 dilution.Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Dot Blot
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Publication Reference
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Cell-free protein production of a gamma secretase homolog.
Celine Moser, Claudia Muhle-Goll.
Protein Expression and Purification 2024 Mar; 215:106407.
Application：WB, N/A, Cell-free expressed proteins.
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BR2 cell penetrating peptide effectively delivers anti-p21Ras scFv to tumor cells with ganglioside expression for therapy of ras-driven tumor.
Ting Yu, Yingxian Shi, Xinyan Pan, Qiang Feng, Peng Wang, Shuling Song, Lilin Yang, Julun Yang
PLoS One 2022 Jun; 17(6):e0269084.
Application：ELISA, Recombinant Protein.
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Cytokine-induced killer cells carrying recombinant oncolytic adenovirus expressing p21Ras scFv inhibited liver cancer.
Fang Dai, Peng-Bo Zhang, Qiang Feng, Xin-Yan Pan, Shu-Ling Song, Jing Cui, Ju-Lun Yang.
Journal of Cancer 2021 Mar; 12(9):2768.
Application：IHC-P, Human, Mouse, Mouse-bearing Huh7-tumors.
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Inhibition of glioma by adenovirus KGHV500 encoding anti-p21Ras scFv and carried by cytokine-induced killer cells.
Jing Qian, Mo Yang, Qiang Feng, Xin-Yan Pan, Li-Lin Yang and Ju-Lun Yang.
Experimental Biology and Medicine 2021 May; 246(10):1228.
Application：IHC-P, WB-Ti, Mouse, Mouse brain, Mouse heart, Mouse Kidney, Mouse large intestine, Mouse liver, Mouse lung, Mouse pancrease, Mouse small intestine, Mouse spleen, Mouse stomach..
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Recombinant Adenovirus KGHV500 and CIK Cells Codeliver Anti-p21-Ras scFv for the Treatment of Gastric Cancer with Wild-Type Ras Overexpression.
Li X, Li F, Ling L, Li C, Zhong Y.
Molecular Therapy Oncolytics 2018 Oct; 11:90.
Application：IHC-P, WB-Ti, Mouse, Organs, Tumors.
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Cell-free protein production of a gamma secretase homolog.
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