ACTB monoclonal antibody, clone BA3R
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of 20 ug/lane tissue lysate from human (1), mouse (2), rat (3) and rabbit (4). Blotting with ACTB monoclonal antibody, clone BA3R (Cat # MAB9743) at 1:1000 dilution.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of ACTB.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of ACTB.
Host
Mouse
Reactivity
Chicken, Human, Mouse, Rabbit, Rat
Specificity
Recognizes native and denatured forms of beta-Actin (~42 KDa).
Form
Liquid
Purification
Protein A purification
Concentration
1 mg/mL
Isotype
IgG2b
Recommend Usage
Western Blot (1:1000-1:10000)
Immunohistochemistry (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS, pH 7.2 (0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of 20 ug/lane tissue lysate from human (1), mouse (2), rat (3) and rabbit (4). Blotting with ACTB monoclonal antibody, clone BA3R (Cat # MAB9743) at 1:1000 dilution.Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
Dot Blot
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