TUBB monoclonal antibody, clone AT5B2
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: Jurkat cell lysate, Lane 2: A549 cell lysate, Lane 3: A431 cell lysate, Lane 4: HepG2 cell lysate, Lane 5: mouse brain tissue extract.
Immunofluorescence
Immunofluorescence of HaCat cells stained with Hoechst 3342 (Blue) for nucleus staining and with TUBB monoclonal antibody, clone AT5B2 (Cat # MAB9723) at 1 : 1000 dilution with Texas Red (Red).
Immunofluorescence
Immunofluorescence of A431 cells stained with DAPI (Blue) for nucleus staining and monoclonal anti-human beta-Tubulin antibody (1:500) with Alexa Fluor 488 (Green).
Immunofluorescence
Immunofluorescence of Balb/3T3 cells stained with DAPI (Blue) for nucleus staining and monoclonal anti-human beta-Tubulin antibody (1:200) with Alexa Fluor 488 (Green).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant TUBB.
Immunogen
Recombinant protein corresponding to amino acids 1-445 of human TUBB.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Concentration
1 mg/mL
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: Jurkat cell lysate, Lane 2: A549 cell lysate, Lane 3: A431 cell lysate, Lane 4: HepG2 cell lysate, Lane 5: mouse brain tissue extract.Immunocytochemistry
Immunofluorescence
Immunofluorescence of HaCat cells stained with Hoechst 3342 (Blue) for nucleus staining and with TUBB monoclonal antibody, clone AT5B2 (Cat # MAB9723) at 1 : 1000 dilution with Texas Red (Red).Immunofluorescence
Immunofluorescence of A431 cells stained with DAPI (Blue) for nucleus staining and monoclonal anti-human beta-Tubulin antibody (1:500) with Alexa Fluor 488 (Green).Immunofluorescence
Immunofluorescence of Balb/3T3 cells stained with DAPI (Blue) for nucleus staining and monoclonal anti-human beta-Tubulin antibody (1:200) with Alexa Fluor 488 (Green).Enzyme-linked Immunoabsorbent Assay
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Gene Info — TUBB
Entrez GeneID
203068Protein Accession#
NP_821080Gene Name
TUBB
Gene Alias
M40, MGC117247, MGC16435, OK/SW-cl.56, TUBB1, TUBB5
Gene Description
tubulin, beta
Omim ID
191130Gene Ontology
HyperlinkGene Summary
O
Other Designations
OTTHUMP00000029069|beta 5-tubulin|beta Ib tubulin|beta-4 tubulin|tubulin beta polypeptide|tubulin beta-1 chain|tubulin beta-5 chain|tubulin, beta polypeptide
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Interactome
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Pathway
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Disease
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