Western blot analysis of tissue lysates (35 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with EIF5A monoclonal antibody, clone AT2E9 (Cat # MAB9715) at 1 : 500 dilution. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : mouse lung. Lane 2 : mouse kidney.
Immunofluorescence of human HeLa cells stained with Hoechst 33342 (Blue) for chromatin staining and EIF5A monoclonal antibody, clone AT2E9 (Cat # MAB9715) (1 : 500) dilution with Texas Red (Red).