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Last updated: 2017/9/24

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EIF5A monoclonal antibody, clone AT2E9

  • Catalog # : MAB9715
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant EIF5A.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 1-154 of human EIF5A.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Concentration:
  • 1 mg/mL
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • ELISA (1:250)
    Western Blot (1:250-1:500)
    Immunofluorescence (1:250-1:500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot analysis of tissue lysates (35 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with EIF5A monoclonal antibody, clone AT2E9 (Cat # MAB9715) at 1 : 500 dilution. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
    Lane 1 : mouse lung.
    Lane 2 : mouse kidney.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence of human HeLa cells stained with Hoechst 33342 (Blue) for chromatin staining and EIF5A monoclonal antibody, clone AT2E9 (Cat # MAB9715) (1 : 500) dilution with Texas Red (Red).
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 1984
  • Gene Name:
  • EIF5A
  • Gene Alias:
  • EIF-5A,EIF5A1,MGC104255,MGC99547,eIF5AI
  • Gene Description:
  • eukaryotic translation initiation factor 5A
  • Other Designations:
  • OTTHUMP00000128384,uORF A
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