Ahi1 monoclonal antibody, clone 645s3
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More Files
- More Functions
Western Blot (Recombinant protein)
Immunoprecipitation and Western blot analysis in Lane 1: parental BaF3 cells, Lane 2: BaF3+BCR-ABL (BaF3 cells transduced with BCR-ABL), Lane 3: BaF3+BCR-ABL+mAhi-I (M1, BaF3 cells transduced with both BCR-ABL and mouse Ahi-1, M1 is a clonal cell line), Lane 4: BaF3+BCR-ABL+mAhi-1 (C2, BaF3 cells transduced with both BCR-ABL and mouse Ahi-1, C2 is a clonal cell line) with Ahi1 monoclonal antibody, clone 645s3 (Cat # MAB8962).
Immunofluorescence
Immunofluorescence analysis in Ba/F3 cells with Ahi1 monoclonal antibody, clone 645s3( Cat # MAB8962 ) at 1 : 200 dilution.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant Ahi1.
Amount
100 uL
Immunogen
Recombinant protein corresponding to C-terminus of mouse Ahi-1.
Host
Mouse
Reactivity
Mouse
Specificity
This antibody only specifically reacts with murine Ahi-1.
Form
Liquid
Purification
Affinity purification
Concentration
1 ug/uL
Isotype
IgG1
Recommend Usage
Western Blot (1:1000-1:2000)
Immunoprecipitation (1-2 ug/200-800 ug protein)
IHC (1:200-1:1000)
The optimal working dilution should be determined.Storage Buffer
In PBS, pH 7.4 (0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Recombinant protein)
Immunoprecipitation and Western blot analysis in Lane 1: parental BaF3 cells, Lane 2: BaF3+BCR-ABL (BaF3 cells transduced with BCR-ABL), Lane 3: BaF3+BCR-ABL+mAhi-I (M1, BaF3 cells transduced with both BCR-ABL and mouse Ahi-1, M1 is a clonal cell line), Lane 4: BaF3+BCR-ABL+mAhi-1 (C2, BaF3 cells transduced with both BCR-ABL and mouse Ahi-1, C2 is a clonal cell line) with Ahi1 monoclonal antibody, clone 645s3 (Cat # MAB8962).Immunohistochemistry
Immunofluorescence
Immunofluorescence analysis in Ba/F3 cells with Ahi1 monoclonal antibody, clone 645s3( Cat # MAB8962 ) at 1 : 200 dilution.Immunoprecipitation
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Gene Info — Ahi1
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