MBP monoclonal antibody, clone QD-9
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of MBP monoclonal antibody, clone QD-9 (Cat # MAB8817) in extracts of multiple systems atrophy (MSA) brain homogenates. The antibody detect a major 18.5 kd band and a weaker 17.2 kd band.
Immunohistochemistry
Double staining with MBP monoclonal antibody, clone QD-9 (Cat # MAB8817) and anti-ubiquitin in a multiple system atrophy (MSA) case (moderately affected cerebella medulla).
The distribution of Cat # MAB8817 (purple) is different from that of ubiquinated glial cytoplasmic inclusions (GCIs, brown), medulla (case M6). Ubiquinated GCIs and Cat # MAB8817 positive fibers are detected. Note that Cat # MAB8817 does not stain the GCIs.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of MBP.
Immunogen
A synthetic peptide corresponding to amino acids 215-221 of human MBP.
Sequence
QDENPVV
Host
Mouse
Reactivity
Human
Specificity
The QD-9 antibody can specifically detect demyelinating lesions in brains with multiple sclerosis, multiple system atrophy (MSA), as well as infarcted brains. The QD-9 antibody is, therefore, a very useful tool for detecting demyelination but not myelin in normal brain.
Form
Liquid
Concentration
1 ug/uL
Isotype
IgG
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS, pH 7.4 (0.05% sodium azide).
Storage Instruction
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, store at -20°C or below.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of MBP monoclonal antibody, clone QD-9 (Cat # MAB8817) in extracts of multiple systems atrophy (MSA) brain homogenates. The antibody detect a major 18.5 kd band and a weaker 17.2 kd band.Immunohistochemistry
Double staining with MBP monoclonal antibody, clone QD-9 (Cat # MAB8817) and anti-ubiquitin in a multiple system atrophy (MSA) case (moderately affected cerebella medulla).
The distribution of Cat # MAB8817 (purple) is different from that of ubiquinated glial cytoplasmic inclusions (GCIs, brown), medulla (case M6). Ubiquinated GCIs and Cat # MAB8817 positive fibers are detected. Note that Cat # MAB8817 does not stain the GCIs. -
Gene Info — MBP
Entrez GeneID
4155Gene Name
MBP
Gene Alias
MGC99675
Gene Description
myelin basic protein
Omim ID
159430Gene Ontology
HyperlinkGene Summary
The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. [provided by RefSeq
Other Designations
Golli-mbp|OTTHUMP00000174383|OTTHUMP00000174384|OTTHUMP00000174385|OTTHUMP00000174386
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Interactome
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Disease
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Publication Reference
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Early white matter pathology in the fornix of the limbic system in Huntington disease.
Sanaz Gabery, Jing Eugene Kwa, Rachel Y Cheong, Barbara Baldo, Costanza Ferrari Bardile, Brendan Tan, Catriona McLean, Nellie Georgiou-Karistianis, Govinda R Poudel, Glenda Halliday, Mahmoud A Pouladi, Åsa Petersén.
Acta Neuropathologica 2021 Nov; 142(5):791.
Application：WB-Ti, Human, Human fornix.
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Tissue Transglutaminase contributes to myelin phagocytosis in interleukin-4-treated human monocyte-derived macrophages.
Sestito C, Brevé JJP, Bol JGJM, Wilhelmus MMM, Drukarch B, van Dam AM.
Cytokine 2020 Apr; 128:155024.
Application：IF, Human, THP-1 cells.
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Early white matter pathology in the fornix of the limbic system in Huntington disease.
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