Western blot analysis of MBP monoclonal antibody, clone QD-9 (Cat # MAB8817) in multiple systems atrophy (MSA) brain homogenates.
Double staining with MBP monoclonal antibody, clone QD-9 (Cat # MAB8817) and anti-ubiquitin in a multiple system atrophy (MSA) case (moderately affected cerebella medulla). The distribution of Cat # MAB8817 (purple) is different from that of ubiquinated glial cytoplasmic inclusions (GCIs, brown), medulla (case M6). Ubiquinated GCIs and Cat # MAB8817 positive fibers are detected. Note that Cat # MAB8817 does not stain the GCIs.
The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. [provided by RefSeq